I wanted to know if anyone has any experience with this rapid tissue clearing technique, link to the paper -
https://www.nature.com/articles/s41598-018-20306-3
It looks very promising, but I am unsure about mounting sections in a formamide medium.
My advice is, beware of any new techniques in your experiment. Even if the old techniques looks a bit more time-consuming, stick with the tried and tested methods. You never know when new problems will be detected with these new techniques.
Regards
Hi, I wanted to clear 150um brain slices and tested three methods all published btw. 90% glycerol, RTF and 47% 2,2'-thioldiethanol (TDE). Clearing takes a couple of hours at RT. The best in my hands was TDE method. I can give your the link to the articles if needed. Good luck.
I'm tying it right now. Im not able to get the clearing capacity they present in the paper. Even a small tissue, like isolated hippocampus, is not cleared very well. I tried to extend the times, but nothing really works. It works well with embryonal brain though. Maybe tissues from max P7 would work but for adult brains I would recommend another clearing method.
Anyway, tomorrow I plan to go check it on light sheet microscope so I will update whether it is usable or not.
See, I told you. Any new method needs to be tried and tested before it is ready to be used in general. Trouble shooting new methods is a pain in the neck. Most of the time you can not do it given the constraints of grant money, lab time etc.
On the other hand, if you can really trouble shoot and make the new method applicable to your particular case then may be you will have a new paper. Now, that would be really good news!
Regards
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