Peter Búran

3 Questions 1 Answers 0 Followers

Questions related from Peter Búran

Is it necessary to use magnesium-free buffer while measuring NMDAr activity?

Hi there! I'm trying to perform an experiment to prove one specific protein interacts and activates NMDAr. For this purpose i'm trying to use Fura-2AM dye to see changes in Ca2+ levels inside the...

07 June 2022 4,978 1 View

How long does the pores in cell membrane last during in utero electroporation?

Hi, I have an experiment in which I have to electroporate embryos followed by injection some antibodies in the brain. Unfortunately I don't know how long should I wait with the injection after...

26 November 2018 6,834 0 View

What might cause high non-specific reactivity on western blot using human sera as a primary antibody?

Hello, I'm currently testing some human sera on reactivity to specific proteins using western blot. We are expecting to find some reactivity in group of interest, but the problem is we see fairly...

14 September 2018 4,354 1 View