Dear Sukaina,

Glucose uptake usually is mostly measured with intact cells, not with lysates. Do you mean that you are perfusing an organ with the glucose analogue (2-DG, or inject it into an animal) and then want to measure how much of the molecule has been taken up by the tissue, depending on a stimulus like e.g. an insulin sensitizer and insulin?

I had a quick look at the AAT assay page at https://www.aatbio.com/products/screen-quest-fluorimetric-glucose-uptake-assay-kit. As I understand it, is has been designed for cells, not for tissue, so there is probably a lot of optimization to do in order to obtain usable results. The 2-DG amount and concentration is probably important, as many tissues are competing for it. So the first thing I'd do is asking the manufacturer for support, maybe they have tried it already and give you some clues.

Dear Wolfgang Schechinger

Thank you for the response.

Yes, I am checking their uptake difference after exposure to a solution. I know similar kits have been tried with what I want to do. They have used the Promo-Cell Kits. Since I am not able to get the same kit, I am trying different manufacturers.

I did speak to them, they said it would work with tissue lysates. Theoretically, they should work, I just wanted to check if anyone has tried with the same manufacturer.