Which is the best way to track down neurogenesis spatiotemporally in my samples?

i would like to know the best way to track down neurogenesis spatiotemporally in my samples, please suggest the best method? which is the best method?

02 March 2015 8,993 6 View

Why is the nucleus not getting stained when Davidson's Fixed tissue are used for immunofluorescence staining?

There are papers stating it is possible to stain nucleus but I'm having difficulties. Please help me. Thank you all in advance.

01 February 2015 1,250 5 View

Can anyone give away a small aliquot of anti hyperphosphorylated Tau antibody?

I am in need of hyperphosphorylated tau antibody,

31 December 2014 7,009 1 View

Does anybody have study materials for Electron microscopy morphology studies of mitochondria?

In order to study ROS and mitochondria morphology I require to study these things Thank you guys in advance.

31 December 2014 5,037 5 View

Are there any sagittal brain atlases?

I am interested in sagittally representing rat brain structure, I was woundering if there were brain atlases for that. Please let me know if there are any?

11 December 2014 2,155 5 View

Do dead neurons take up golgi staining?

Additionally, I find varicosities in some neurons. I am puzzled as to what they amount to?

11 December 2014 9,995 15 View

Can anyone provide me with a method for conducting histological investigations, I wish to study Ischemic events in neonatal rats?

09 October 2014 6,273 3 View

Are there behavioural tests for visual acuity in rodents?

For ischaemic condition there are retinal disturbances. Are there behavioural tests for visual acuity in rodents?

07 August 2012 6,378 3 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View