Which is the best way to track down neurogenesis spatiotemporally in my samples?

i would like to know the best way to track down neurogenesis spatiotemporally in my samples, please suggest the best method? which is the best method?

02 March 2015 8,993 6 View

Why is the nucleus not getting stained when Davidson's Fixed tissue are used for immunofluorescence staining?

There are papers stating it is possible to stain nucleus but I'm having difficulties. Please help me. Thank you all in advance.

01 February 2015 1,250 5 View

Does anybody have study materials for Electron microscopy morphology studies of mitochondria?

In order to study ROS and mitochondria morphology I require to study these things Thank you guys in advance.

31 December 2014 5,037 5 View

Has anyone tried Golgi staining of rat retina?

I was wondering what sort of structures could be appreciated in a rat retina through Golgi staining.

31 December 2014 486 1 View

Are there any sagittal brain atlases?

I am interested in sagittally representing rat brain structure, I was woundering if there were brain atlases for that. Please let me know if there are any?

11 December 2014 2,155 5 View

Do dead neurons take up golgi staining?

Additionally, I find varicosities in some neurons. I am puzzled as to what they amount to?

11 December 2014 9,995 15 View

Can anyone provide me with a method for conducting histological investigations, I wish to study Ischemic events in neonatal rats?

09 October 2014 6,273 3 View

Are there behavioural tests for visual acuity in rodents?

For ischaemic condition there are retinal disturbances. Are there behavioural tests for visual acuity in rodents?

07 August 2012 6,378 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View