Which is the best way to track down neurogenesis spatiotemporally in my samples?

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Alexandre Hiroaki Kihara

I would say BrdU labeling. Do you know this method?

best,

Jatin Tulsulkar

Yogesh: For Brdu protocol you need to inject your animals initially with Brdu and continue dosing until u sacrifice. If you already got the tissues the only thing I can suggest is use of PCNA or Ki67 along with NeuN antibody.

Fatiha Chigr

As said Christopher, you may specify what do you want really, analyzing your results of neurogenesis or to perform neurogenesis. If the second option, you want, some answers have been given above, notably concerning the use of BrdU and if you are interested I can give you more informations.

Yogesh Kanna Sathyamoorthy

i would like to visualize neurogenesis after stroke and see its consequences!

Its a rat model of stroke and trying to treat it with a compound and wishing to look at the status of neurogenesis.

thank you all in advance

OK ! You have to use 3 animal groups : Control, Stroke group and Stroke-treated group. You have to administrate BrdU 50mg/Kg 2X/day (12h between the two IP injections) for 5 days) during the same period for the 3 groups.

thank you all