I’m planning to extract bacterial LPS. I read about various methods for western blot of LPS and would like some advice about recommended techniques. I’m looking for the most efficient method, as cost and time are limiting factors. A protocol would be useful. Thanks.
Vjollca Konjufca
You can just spot purified LPS on a membrane, let it dry for 15-20 min, wash the membrane, block it, then probe it with anti-LPS antibodies. We use this method routinely for detecting particle-conjugated LPS. However, we are working with already purified "commercial-grade" LPS. If you worry about other impurities in your purified LPS, this method is not the best.
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Alaa AbdulAziz Abed
spot purified LPS on a membrane, then probe it with anti-LPS antibodies
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