Has anyone tried Direct-zol RNA extraction kit by Zymo Research?

More Umberto Palatini's questions See All

Opionions on MinION by Oxford Nanopore

Hi, is anybody using, or has tried, Oxford Nanopore MinION for pathogen analysis and identification, microbial metagenomic and similar tasks?I knew that it wasn't very precise and gave a read...

14 August 2017 3,331 2 View

A good kit for DNA extraction from Agarose Gel?

Hi, I have a primer which produces multiple amplicons, so i observe multiple bands on gel. I was expecting that, and i would like to cut the bands, extract DNA, and use it as template for another...

18 July 2017 2,014 38 View

How can I manually calculate the DNA integrity Number (DIN)?

I have data for DNA quality obtained with Agilent Bioanalyzer DNA HS kit but I don't have the DIN score. Because the runs were performer in another laboratory I can't use the software. How can I...

24 November 2016 8,297 1 View

Is anyone familiar with strength tests in obesity?

I am going to implement an existing aerobic program on obese adults with strength exercises. The course will provide the test protocol for the evaluation of the strength of certain muscle groups,...

07 February 2015 8,835 2 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Nathanael Pruett

I first started using the Direct-zol kit when it was first offered and have found it to yield excellent RNA (including miRNA). The on-column DNase treatment is incredibly convenient, and this kit really is fast. I typically get a yield of ~10µg of total RNA from 2 million cultured cells.