I'm struggling to find resources or an appropriate method of extraction that can break the beta sheets and still have viable DNA for PCR reactions. Any help or references would be greatly appreciated. The current method of various Qiagen kits do not successfully break the beta sheets, but rather just the alpha helix, which is only about 30% of my samples. SDS, pressure, heat, none are giving up viable DNA. I have been searching for over a year and found one resource on beta sheet keratin and it's not very helpful.

Thanks!

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