There are a couple of papers which show the use of this kit on such samples, but we haven't been able to make this kit work on our CINII samples. We have tried the solutions provided in the kit for antigen retrieval and other generic antigen retrieval methods with the antibody but none worked. The tissue always come out with background, when compared to a secondary only control. Does anyone have any suggestions? Also, please note that this kit was purchased 3 years ago but didn't work at the time it was bought either.
http://www.nature.com/emm/journal/v39/n5/abs/emm200768a.html
Hi Megha,
have to be in tissue? you could not use samples in liquid-based cytology?
The kits: PreTect ™ HPV-Proofer, Norchip AS, Klokkarstua, Norway and NucliSENS EasyQ®, BioMerieux SA, France, works well for this.
It is also possible to use OncoTect ™ HPV E6, E7 mRNA Kit of IncellDx, which uses the technique of flow cytometry. I will use this in my research.
I have helped.
Best regards
- Badges
- Science topic
- Similar topics
- Biological Science
- Immunology
- Antibodies