Megha Budhwani

4 Questions 3 Answers 0 Followers

Questions related from Megha Budhwani

Reed and Muench calculation of EBV titer for in vivo use

Hi All, I am trying to work out EBV titer using the Reed and Muench calculator so that the mice can be injected with 1000 TD50. To titrate, I infected PBMCs from cord blood with 6 dilutions of the...

08 August 2017 6,464 2 View

Has anyone successfully detected HPV16 E7 by using the cervimax kit on formalin-fixed paraffin embedded human tissue samples?

There are a couple of papers which show the use of this kit on such samples, but we haven't been able to make this kit work on our CINII samples. We have tried the solutions provided in the kit...

01 January 2015 4,489 2 View

Has anyone used the SK1 clone CD8 antibody for IHC on formalin fixed paraffin embedded tissue sections?

Its recommended for Flow but has just one old reference for IHC-P. As this is going to be used for very precious human tissue sections, I wanted to be absolutely sure before trying it on our very...

11 November 2014 1,231 2 View

Does storing mouse lymphocytes in liquid nitrogen for long-term use work for proliferation assays?

I am working with in-vitro and in-vivo T-cell proliferation assays and use the cells straight away after isolation. But we have a whole lot of OT-II mice which we might not be able to use due to a...

05 May 2014 2,072 6 View