You can use 2:1 (1N HCl : Acetic acid) solution. Hydrolysis the root until it becomes soft enough to make slides.

Best of luck.....

You have not mentioned which staining technique you are using in this case. Have you tried to do it without hydrolysis? Sometimes hydrolysing the roots separately hinders the staining of roots. It happened with me. I used 9:1 2% Propiono-orcein: 1N HCl mix and this helped.

Dear Sreetama & Chandan

Thank you for your attention. I hydrolyzed the root tips in 1 N NaOH at 60 °C for 17 min, and after washing the roots, I used aceto-iron-haematoxylin to staining the chromosomes.

Dear Dr. Miri

Please following these papers.

https://www.jstage.jst.go.jp/article/cytologia/76/4/76_4_425/_article

Article Chromosome number evolution, biogeography and phylogenetic r...

https://www.jstage.jst.go.jp/article/cytologia1929/43/2/43_2_317/_article

http://dergipark.gov.tr/download/article-file/415591

Article A cytomorphological study in some taxa of the genus Salvia L...

Best wishes

Hello Seied Mehdi Miri

I think maybe use these articles are useful to you.

Sincerely,

Obidjon