Cross-country research: How can I control for the factor country, although I don't have any hypotheses regarding country differences?

Hello, I am currently preparing a preregistered report for experiments in different countries. I am studying motivations/values of students; therefore, I want to conduct several same experiments...

28 January 2021 5,763 3 View

How best can one separate n-hexane-soluble fraction of plant extract that binds strongly to silica gel columns and what are the possible compounds?

I wanted to elute n-hexane-soluble fraction of a plant extract in a silica gel column using different proportions of dichloromethane/n-hexane. The bands separated visibly initially but moved only...

18 January 2021 9,270 3 View

How to desinfect a liquid nitrogen tank?

Hey everyone, we'd like to clean and decontaminate our liquid nitrogen tank before stroreing new cells inside. The tank seems to be made of aluminum. Any recommendations on what disinfectant to...

07 January 2021 1,599 3 View

DIANA FEA 10.2: How do I model deformation supports, so that no tension occurs/is allowed ?

Hello together, I am working on concrete direct shear Tests, where i apply loadsteps of a prescribed Deformation (left deformation). Since i can only apply a Deformation with a support inplace i...

20 January 2020 8,225 3 View

Is HSE06 a good functional?

I'm investigating first row transition metal oxides on diamond surface, optimizing structures and calculating electronic properties (e.g. electron affinity). I was advised that since I'm working...

08 January 2020 8,713 4 View

Quick ('on the Fly') update of MM potentials, MD software with Amber ForceField?

Hello, Could anyone recommend a free MD software (that takes Amber ForceFields) where one can very quickly (on the Fly) update the MM potentials of a set of atoms (and query the coordinates of...

20 November 2019 7,716 1 View

What test shall I use when I want to compare an experimental group at various time points to one control group?

The data are as follows (overview of the design attached): One parameter was measured in the blood of the same patients at various time points and also in a healthy control group at one time point...

15 July 2019 1,382 6 View

Can I use ELISA with not ideal standard curve?

Dear colleagues, I would like to ask you if you have any experience how to use data from ELISA analysis when my standard curve is not as showed in the manual? I have many standard curves that have...

17 April 2019 282 4 View

Does it exist any open repositories of user stories?

For a Machine Learning-based project, I'm looking for repositories containing user stories (and acceptance criteria). This can be issue management systems (e.g., stories in Jira) or plain...

19 December 2018 4,195 7 View

What are possible portable multigas sensing devices?

Hi, I'm currently looking for a portable gas measurement system to measure O3, NO2 and CO at the same time. I already stumbled upon GrayWolf Sense and Aeroqual. Can someone suggest other...

13 September 2018 6,690 2 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View