I'm trying to develop a bunch of microsatellite primers and am trying to use an alternative to the classic Thermo dyes. Has anyone had success with this? I found an unpublished document "MULTIPLEX MICROSATELLITE ANALYSIS WITH 5’ DYE-LABELLED MARKER SETS USING FAM, YAKIMA YELLOW, ATTO 550, ATTO 565, AND ATTO 633 ON AN ABI 3130XL GENETIC ANALYZER" saying it works but that they changed the dye matrix. Does it work with the classic dye set 33?
Of those I have tried only Yakima Yellow and, as I found in general with switching from the main dyes, the peaks were weaker and more difficult to call. Also, incase you want to compare to previous data changing the dyes changes the size of the microsatellite call but likely not the case since you are developing them.
What about the prices? Is it really worth trying this dye set economically?
- Badges
- Science topic
- Similar topics
- Chemistry
- Biochemistry
- Biochemical Techniques
- Blotting Techniques
- Immunoblotting
- Dyes