Has anyone proposed a fat mass or adiposity cut-off point to define obesity in mice?

More José Fernández-Calleja's questions See All

Why do open and free science in a world where science is not open and free?

Because I have realized that the world tends more and more to do open and free science and there is a trend more and more to choose free databases, free tools and open access platforms.

01 August 2024 10,046 1 View

How can I use DFT-D3 correction ( Siesta version 5.0.1)?

Dear Siesta Users, I installed Siesta 5.0.1 and I want to use Grimme's D3 correction. According to the 5.0.1 manual, I could use a few parameters to include the D3 correction in the...

27 July 2024 5,748 0 View

Comparison of Methanol and ethanol treatment on collagen based scaffolds?

Kindly share a research paper that explains the effect of Methanol and ethanol treatment on collagen based scaffolds. How it increases porosity and swelling. Kindly help me find a paper. Thank you

24 July 2024 3,937 0 View

How to represent Annexin V apoptosis study data in publications?

Hi, I have performed an Annexin V apoptosis study using flow cytometry and got quite good results. I doubt how to represent the quadrant percentages in the final bar graphs. Some papers only show...

13 July 2024 356 3 View

¿Where to buy the Aspergillus oryzae NSAR1 strain?

Greetings: I am looking to buy/obtain the auxotrophic strain Aspergillus oryzae NSAR1, but I just have found one culture collection from Japan but haven't received any answer. Could you provide...

11 July 2024 6,847 0 View

In silico techniques for identification of inhibitor/activator/substrate activity?

Hello dear researchers, I would like to know about available in silico tests that could predict the activity of ligands (small molecules) against the target protein. I have performed docking...

08 July 2024 4,724 4 View

Is seti need to be fixed in radio colimated?

seti or search of intelgenece articial nee to be fixed due to masss solar systems outhere thta expelled laser beam light ollimated wel beign so is neede to modificated the seti in a new device of...

21 June 2024 5,059 1 View

Best method for extracting RNA from low biomass of Ectocarpus (filamentous brown algae)?

Hello everyone! I am currently working with the model organism Ectocarpus (filamentous brown algae). Our team is trying to standardize a protocol for RNA extraction using low biomass (around 10...

18 May 2024 3,862 1 View

Problems in measuring TG/DTG, Different results for the same sample, how to fix it?

TG measurements on the Hitachi STA7300 equipment started to have different results for the same material after some time of use Some tests have already been carried out to try to correct it: Gas...

17 May 2024 7,438 4 View

¿quiero tener tesis sobre el equilibrio en la educación física?

tener una relacion el ejercicio físico con actividades de equilibrio

16 May 2024 5,290 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How can reinforcement learning algorithms be effectively applied to study decision-making processes in neuroscience?

I am interested in exploring the intersection of neuroscience and AI, particularly through reinforcement learning for understanding decision-making in the brain. I also want to integrate...

17 July 2024 1,225 0 View

FACS markers to characterize rat bone marrow-derived macrophages?

Dear all, I am looking for FACS markers to characterize my rat bone marrow-derived macrophages (M0). They were differentiated from monocytes with M-CSF. I am considering to use CD11b/c and...

15 July 2024 4,615 1 View

How do the complex dynamics of the oral microbiome interact with the host immune system affect periodontal disease development and treatment response?

This research question explores the complex dynamics of interactions between the oral microbiome and the host immune system, particularly in the development and treatment response of periodontal...

09 July 2024 7,922 1 View

What is the association and mechanism between bacterial diversity in the oral microenvironment and clinical manifestations of periodontal disease?

This research question explores the complex relationship between bacterial diversity in the oral microenvironment and the clinical manifestations of periodontal disease. The oral microenvironment...

09 July 2024 6,210 1 View

Abaqus 2023 - How to access element - scale for implementing a failure criteria with subroutines (UMAT, USDFLD, UEXTERNALDB, ..) ?

Hi all, Second question on the same or so topic. I am working on subroutines (UMAT and others) for Abaqus to implement Tsai-Wu failure criteria for non-composite structures (I work on long...

25 June 2024 2,955 1 View

How to select drugs for repurposing?

Hello everyone, so I am searching for ways to find the method for picking the right drug for repurposing. This repurposing is for existing and new diseases. This is very important, please help.

23 June 2024 7,730 2 View

What is the best protocol for bone marrow cell culture? Is it critical to isolate the mesenchyme cells in advance?

I need to set a protocol to culture the bone marrow cell. I appreciate it if anyone help me.

22 June 2024 7,562 2 View

Use of agency vs. google-translation for translating non-english qualitative data?

Hi, As part of a miltilevel study examining the impact of steroid toxicity in patients with different rheumatic diseases (see here: https://vasup.ndorms.ox.ac.uk/) we collected data from the UK...

17 June 2024 4,016 4 View

Have pathomorphological studies of the lungs been performed in patients with desminopathy?

It is known that patients with desminopathy often die from pneumonia. Have pathomorphological studies of the lungs been performed in patients with desminopathy?

15 June 2024 1,355 7 View

Dina Mostafa

In my case, I consider mice obese after HFD for 3 month if there body weight is 50gm or more. I hope this answers your question.

José Fernández-Calleja

Thank you for your answer Dina, it certainly helps in my research. Best wishes, José

Kamel Charradi

I confirme the answer of Dina Mostafa