Looking to perform intracellular staining on HUVECs in 96-well plates, but need to wash off excess permeabilization buffer and antibodies, but don't want to take them out of the wells if possible.
Matej Vicen
If you want to perform immunocytochemistry intracellular staining you need to fix the cells first (acetone or paraformaldehyd fixation according to your stain) this fixation will secure your cells in the wells so you dont need to be afraid of removing the cells out of the plate.
Which method do you want to use the cells for? Maybe I could help more if you provide more information about your experiment.
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