Mark Ross

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Questions related from Mark Ross

Has anyone performed intracellular stainon on Human Umbilical Vein Endothelial Cells on a 96-well plate? Issues regarding washing excess buffer/mAb?

Looking to perform intracellular staining on HUVECs in 96-well plates, but need to wash off excess permeabilization buffer and antibodies, but don't want to take them out of the wells if possible.

07 July 2019 5,303 1 View

Has anybody used phospho-antibodies for phosphorylation status of proteins/enzymes by intracellular flow cytometry?

Looking to use phospho-antibody (Ser177) for p-eNOS staining by flow cytometry in human umbilical vein endothelial cells.

06 June 2019 7,577 3 View