What did you do with it? Has it worked properly for you?
Other than Sparids
27 June 2024 1,809 0 View
1. Vachellia drepanolobium 2. Vachellia horrida 3. Vachellia karroo 4. Vachellia nilotica 5. Vachellia oerfota 6. Senegalia polyacantha ? [email protected] +2348061166247 Thank you.
05 June 2024 4,858 1 View
Hello, I'm writing Python scripts for Abaqus and I'm facing a problem. I need to change the coordinate system in a .odb file before extracting data but I'm stuck. I can create my coordinate system...
30 May 2024 7,431 3 View
Recently i did PFGE and WGS comparison with the same set of E coli strains. In my mind, if PFGE give two different pulsotypes, it is sure that strains are not the same. But when i did WGS, i...
12 May 2024 8,107 2 View
Dear community, I want to perform an analysis of the risilin localization within the legs of myriapods and would like to visualize the localization of the resilin components in different...
07 May 2024 9,160 3 View
I would like to use the "create.matrix" function (R package "fossil") to create a matrix of taxa, localities, time and abundance. In the function, time is specified by the arguments "time.col" (=...
28 April 2024 3,545 2 View
I am trying to do a golden gate cloning but the ligation-digestion seems to be failing
01 April 2024 6,416 2 View
Hello, What would be the best procedure right after extracting brain samples from mice, if we plan to analyze the samples later ? Would it be best to just directly flash freeze the brain...
28 February 2024 7,384 1 View
Right now I'm working on a senior project in combinatorial game theory - I've read Knuth's book "Surreal Numbers" and I've started reading "Winning Ways for your Mathematical Plays" by Conway,...
20 February 2024 1,254 1 View
What is the Current situation with poverty and inequality in Bangladesh? What are the potential sources/causes of poverty and inequality in Bangladesh? What are the consequences poverty and...
17 February 2024 4,166 0 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View
In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,076 3 View
I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?
23 July 2024 1,353 4 View
hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...
21 July 2024 8,660 1 View
I need to know the approximate size of my target protein for Negative-stain EM. I have an alpha-fold predicted structure of the protein. I do not see any turtorial on internet for estimating the...
21 July 2024 5,732 3 View