I would not buy Roche official 96-wells, because are too expensive and to find other company that produce compatible plate.
see: PCR PLATE COMPATIBILITY CHART
http://www.usascientific.com/pdflit/pcrcompat.pdf
Hey Francesca, I've been using the hard shell plates from biorad on our LC480 an they work brilliantly.
Hard-Shell 480 PCR Plates
Hi, I would need some help in understanding the SEM output. I run a latent change score analysis to test the change score of a variable of interest at two-time points (repeated measure) PLD1 and...
05 January 2021 3,367 2 View
Dear all, I'm trying to transfect HeLa cells with lipofectamine 3000 in a 10 cm dish. Do someone of you have a protocol to share? I still examined the table for scaling up or down lipo3000 but it...
13 October 2020 2,926 3 View
Hi everyone, I am looking for suggestions to store biological samples in the field for endocrinology analyses, where I will not have electricity. One of the options we are evaluating is a solar...
01 September 2020 3,766 3 View
Hi everyone! I have a doubt and I hope there is someone who can give me an advice. I am modeling a MEMS gyroscope for my Master thesis and I am starting with a very basic structural design. My...
20 August 2020 9,524 5 View
Recently I'm trying to edit a pure component mass density and heat capacity of an existing component NaNO3 but failed. Could anyone help me on this? Thanks a lot.
17 July 2020 8,800 8 View
Hi! I am currently translating and adaptating a questionnarire about attitudes towards inclusion, and I would like to have suggestions about specific literature in translation and cross-cultural...
23 March 2020 2,630 3 View
01 March 2020 3,206 2 View
hi everybody, i have some troubles with chemokines and cell proliferation. Different papers evidence that CXCL8 (but also CXCL12, CCL2 and other chemokines) are able to promote cancer cell...
03 February 2020 214 4 View
Hi, we would like to analyze time sequences images of calcium imaging assay performed with Fluo4-AM in imagej. Is there any plugin that we could install to do this? Do you have any kind of...
27 January 2020 2,665 1 View
I have to perform RNA extraction with TRIzol LS and I just realized that we have chloroform stabilized with ethanol. Is that ok? And should chloroform and isopropanol be "molecular biology...
30 September 2019 4,647 5 View
Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...
03 March 2021 687 1 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...
27 February 2021 547 3 View
If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...
26 February 2021 3,526 3 View
Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...
26 February 2021 4,777 4 View
Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...
26 February 2021 5,029 2 View