Nathan Kieswetter

3 Questions 2 Answers 0 Followers

Questions related from Nathan Kieswetter

Low RNA yields from healthy patient CD14+ cells?

Hey, So I have question regarding total RNA extracted from CD14+ cells in comparison to THP-1s. When working with approximately 2 million CD14+ cells, I typically end up with about 1-1.5 (often...

14 July 2016 708 6 View

At what concentration should THP-1 cells be frozen down at to encourage vigor and viability post thawing?

Hey All, I just wanted to check if anyone has observed differences (vigor and viability) in thawed THP-1 cells at varying initial freezing concentrations (Cell/ml). In general I freeze down...

07 April 2015 575 4 View

Do I need to reverse complement my reverse primer?

Hey, I've a designed a series of primers against ref seq. sequences using Primer-Blast (which uses Primer 3 software) for qPCR. Once I'm happy with the primers, I get an output for the forward and...

16 March 2015 6,430 3 View