I am trying to isolate lymphocytes and/or myeloid cells for FACS analysis from the cohlea of mice p.n. day 4-16. Considering that there is a lot of hematopoietic bone marrow in the temporal bone itself, even though I microdissect the tissue, I get a lot of hematopoietic cells which, naturally, interferes with my data. Does anyone have any experience with this?
Dear Marko,
I have to admit that I'm not experienced with cochlea tissue. What exactly is the problem that prevents you from isolating single lymphocytes or myeloid cells? Are these cell types difficult to distinguish under the microscope? Can you do IHC or immunofluorescence staining to visualize and detect the cells of interest? Or is your microdissection system not precise enough to cut single cells? Which one are you using? Is the laser calibrated well?
Kind regards,
Heidi
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