What is the maximum number of reads per one run with NGS?

Does anybody know how many reads are usually generated with Illumina NextSeq? For sequencing plasmid library (100-150 bp per read), for example.

27 September 2020 799 2 View

Glioblastoma GL-261 cell line not growing s.c?

Hi has anyone had experiance of GL-261 not growing subcutaneously? I have inoculated 10e6 cells in C57BL/6J female mice and the tumors became palpable after a week cca but have subsequently...

19 February 2020 7,543 2 View

Microplastic extraction from fish guts using soluble filters?

Hi there, I want to isolate microplastic particles from fish guts and water samples. My initial idea was to extract guts from selected specimens and then dissolve the whole guts using 10% KOH...

12 February 2020 4,467 8 View

Why are my glucose levels decreasing very rapidly?

Hello, I'm conducting an IPGTT experiment on WT and KO mice (mice which we have shown to have improved glucose tolerance) and I have a serious issue of glucose levels plummeting after 30-60min....

28 September 2018 962 4 View

No title

10 December 2015 1,475 1 View

How can I calculate deflection of steel fiber reinforced concrete beams?

How can I calculate deflection of SFRC beams (ordinary and high strength)? Is there ACI or other proposals?

11 May 2015 4,977 14 View

No title

I need approximately 100 meters of ceramic cord (0,5mm). I need it for attaching extensometer to the tensile specimen when conducting tensile test at elevated temperatures.

10 November 2014 4,572 3 View

What is the best bacterial vector for large inserts (around 50 kb)?

Is it possible to insert 50 Kb of DNA to a bacterial vector and successfully maintain and amplify it in bacteria? If so, what would be the best vector?

12 January 2014 8,832 2 View

Where could I find complete interviews of some convicted Western jihadists?

I am NOT looking for studies on the subject, but actual interviews.

01 January 1970 5,977 2 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

Which is the most convenient manufacturing software for Additive Manufacturing?

Which tools can be used to generate machine codes for additive manufacturing?

02 March 2021 7,220 2 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

Does different dilution factors affect negatively on NH3 reading ?

I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...

27 February 2021 8,483 3 View