Has anyone isolated endosomal membranes?

More Herschel Dhekne's questions See All

How to calculate schmid factors with ATEX?

Can anyone help me with the schmid factor analysis in ATEX. I am a new user of ATEX.

08 September 2021 7,551 2 View

Pressure drop across packed bed

I have packed bed of granules. Bed gets swell when comes in contact with water. I would like to measure pressure drop across packed bed. Water is an fluidising medium. How to do actual measurement...

28 July 2017 864 4 View

How to compute liquid-liquid diffusion coefficient by experiment?

I am interested in experimental measurement of diffusivity of tea brew in water? Could you please explain diffusion cell apparatus for measuring liquid diffusivity.

09 July 2017 2,902 5 View

What do you think is the most urgent research topic related to leaching of pesticides?

I would like to know scope of research in leaching pesticide due to heavy rainfall or spilling of pesticides on the surface. Is there any scope for mathematical modelling of leaching as in...

06 July 2017 7,745 26 View

Can you explain me, Darcy's equation for 3 dimensional flow through packed bed?

I am interested in modelling of TEA BAG infusion kinetics, and I have considered tea bag as packed bed. The final goal is to find out the concentration of tea in fluid(water). Please tell me where...

07 April 2017 496 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Bhuminder Singh

I know of one method published from our lab. It is called Fluorescence activated vesicle sorting. PMID: 18504258

Requires complex FACS instrumentation and expertise though.

Herschel Dhekne

Hi Bhuminder, what a coincidence that you are from Jim Coffey's lab. I have recently received an offer letter for postdoc position in Tyska lab in Vanderbilt. May be we should talk if you have time.