Thank you Chang-Nim. Just one more question are you implying that CDy1 is acutally staining cells that are more metabolically active - rather than for pluripotency?
I myself am having some troubles with the CDy1 dye at the moment. Prof. Chang Young-Tae suggested that the dye might be internalized by a transporter and sequentially stains mitochondria.
The hypothesis of the staining of the more metabolically active cells is interesting and worth a try. As my Internship will end this week i wont be able to do experiments on this behalf, but still i would be very interested in the "mechanism" of this dye