Ronny Fischer

3 Questions 6 Answers 0 Followers

Questions related from Ronny Fischer

How can I Compare/Evaluate pipetting precision among staff members?

I hope all are well. I need the networks help on this one. I am trying to evaluate our staff members' pipetting precision. I know there is the gravimetric approach. But I was thinking about using...

03 April 2022 6,695 3 View

Can i change pH of buffer after cleaving Hyaluronan with Hyaluronidase?

For an experiment i need the small cleavage products of Hyaluronan, meaning that i need especially the activity of Hyal-1 in the product H3884 of Sigma. I guess this is why standard protocols use...

26 April 2017 2,937 2 View

After addition of protease inhibitor protein concentration (Bradford) decreased when sample was centrifuged but increased when filtered. Why is that?

Sample: colon lavage I took 2 times 1ml of the same sample. After that one sample was centrifuged (14000 rpm, 4°C) and the other was filtered (0,4µ pore size). I took 2µl of each sample and...

04 July 2016 1,600 11 View