Is there any databases of sequencing data for AD mouse models?

I am working with the NL-G-F and 5x FAD mice. Are there any sequencing databases publicly available to mine for brain or retina? Thanks!

20 January 2021 6,425 1 View

Does anyone have problems performing CRISPR KO in human primary T cells?

For the past few months, I have been troubleshooting a CRISPR KO of human CD3e in human primary T cells. I got the KO to work one time using 1250 ng TrueCut Cas9 (Thermo) + 250 ng (or 7.5 pmol)...

11 January 2021 6,016 3 View

What are the applications of remote sensing and GIS in transportation, what are the types of sensors you will require?

How do you use Remote sensing and GIS in transportation, what are the types of images you will require, what will be their wavelength, what type of sensors will you require?

20 December 2020 6,375 5 View

Non-biological recognition elements for bacterial sensors?

Hi, I am interested in non-biological recognition elements for bacterial sensors. I am aware of molecularly imprinted polymers but I do not seem to find many other alternatives. I know that many...

18 September 2020 5,894 3 View

Linear Mixed model analysis - assessing baseline covariate?

I am analysing treatment group differences at time 2 (two time points only) data on a psych measure outcome (with five covariates: age, gender, CVD, BMI and F2s), and I am interested in one of the...

25 August 2020 6,904 3 View

Autoclaving Powder Skimmed Milk?

Hello! Does anyone has a effective way to autoclave 10% skimmed milk without caramelization? I tried 121C for 15min (in the powder form) but I am struggling with the color which is undesirable....

30 June 2020 4,601 3 View

Technique to estimate the amount of nanoparticles in a coating?

Hi everyone, I am coating stainless steel with 10%PLA and Nanoparticles, and I am trying to find out techniques that I can use to characterize the coating surface aside from SEM and to quantify...

08 June 2020 2,293 7 View

How to inactivate SARS-Cov-2 in tissue for spatial transcriptomics?

We want to run 10x Genomic Visium spatial transcriptomics on lung tissue from COVID-19+ patients. How can we inactivate the virus so it's safe to work with the tissue at BSL2? The first step is...

28 April 2020 4,704 3 View

How can one perform spin coating of stainless steel 316L with a polylactide-chloroform solution?

I am trying to do for my thesis a uniform homogeneous coating with PLA (polylactic acid with 1.05 dl/ g viscosity) in Stainless steel 316L medical grade via spin coating, but I haven't been able...

07 April 2020 8,556 3 View

What is the mutation rate in the mtDNA of the Lepidoptera?

I want to determine the phylogenetic age of the members of the Lepidoptera (Sphingidae) based on mtDNA, using BEAST software; But I do not have a good indicator. Please what is mutation rate in...

28 March 2020 1,559 3 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

Are there packages (R or Python) for simple models (bucket?) to assess soil water content?

Hi everyone. I am researching droguht effects on soil water drought. I am looking for already implemented functions in R or Python to estimate soil water content from precipitation,...

21 February 2021 2,084 1 View

Which type of loading is to be used in dynamic analysis of Machien foundation?

Hello Everyone, Hope you all are doing well. I'm trying to simulate a machine foundation in ABAQUS with a geo-cell reinforced soil bed. Im totally confused to which type of loading would be...

21 February 2021 4,388 3 View

Measurement of water retention in organic substrates without a pressure plate?

Hello, I'm trying to measure the physical and hydrological properties of organic substrate media. Currently, I'm referring The book Soil sampling and Methods of Analysis by Carter and Gregorich....

09 February 2021 3,233 8 View

Where can I find FDI data by sector/industry for Asian countries like India and China for the last 30 years?

Common databanks like UNCTAD, OECD, WorldBank and ASEAN Website did not have the data for the last 30 years.

09 February 2021 4,701 4 View

What will happen (quality or quantity wise) if we transplant 'Aerobic Rice' in puddled condition?

I want to know about the consequences of aerobic rice (both quality and quantity-wise) when transplanted or sown in puddled condition?

08 February 2021 2,343 6 View

Do there exist stand-alone permanently in-ground soil-moisture sensors with an installed GSM module, for outdoor use?

Am searching for a stand-alone permanently in-ground soil-moisture sensor with an installed GSM module, for outdoor use. The idea is for the sensor to send a moisture reading via a cellphone text...

04 February 2021 2,047 4 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View

What is the maximum storage time for samples used in soil enzyme assays?

01 February 2021 4,616 3 View

From where can I get details of following terms - Typic Fluvaquents, Aeric and Typic Epiaquepts?

I Have got a soil map in which following terms are given - Vertic Ustochrepts, Typic Ustochrepts,Typic Fluvaquents, Aeric and Typic Epiaquepts, Aquic and Typic Ustipsamments, Typic Ustorthents....

26 January 2021 8,412 5 View