I have applied the squash technique for staining with basic fuchsin, but it didn't stain.
I repeated the same procedures using onion and maize root tips; only onion root tip was stained. Anyone know any solution for this problem?
@Please try with 2% acetocarmine stain. Sometimes 1% stain is lighter and give no contrast. Warn the slide gently. Also check your fixation procedure. Pre-treatments of the material are important. Please take care of that part as well. What you have observed in the slide so far?
Please check the link given below. It may help you.
http://www.caryologia.unifi.it/past_volumes/55_2/Caryologia_55_2_115-119.pdf
Regards
Dr/ Bryan Kindiger, thank you for your reply.
I will try the UV lamp treatment for the stain
Dr/ Pablo Bolaños-Villegas, yes I do.I tried from 5min to30 min
You can try Improved phenol fuchsin, it will stain the sample within 5min.
We use Haematoxylene as a primary stain after keeping 2-3 minutes with iron -alum solution for 3 mins so that stain will sustain for a relatively longer time.
Are you hydrolyze the root tip with HCl? HCl will affect the staining if it still stay in tissues or cells. So, at my experience, maybe you can get better staining result after wash the tips more times with distilled water.
My suggestion is that you should fix roots in acetocarmine for 48 h and then squash at 45% acetic acid.
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