I am trying to assess the viability of cells embedded in 2% Sodium alginate hydrogel, however the cell viability is very low (20-30%) after 24 hours. Can anyone provide a solution to this issue?
What are using for cell viability assay? Within 24hr it won't adapt to the environment, so it wont show rapid proliferation. Give some time. We got an increase in viability after 72hr. Well, our was WJMSCs.
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Saurabh Mandal, I am using Calcein AM and PI staining. I realized that the proliferation rate is higher after 24 hours. Thanks a lot for the answers Omkar Singh Kushwaha and Saurabh Mandal