Hi,
I have been using the KSV Langmuir Trough and LayerBuilder for a long time now. Recently, we had to move the instrument due to renovations and I thought of calibrating it once again. We followed exactly all the procedures that are recommended in the manual, taking utmost care at every step. The supplied calibrated weight (metal disc) was measured on a sensitive analytical balance (258.82 mg) and used for calibration of the electrobalance. However, after calibration, the weight difference is almost 2.38 mg. When I zero the balance and measure the weight of the supplied calibrated weight, it shows as 261.20 mg in the Manual Control window instead of 258.82 mg.
For those who have worked with this instrument, can you provide me with any details on how you tackled this issue?
Also, do you think a 0.91% error in the weight is quite a lot for surface pressure measurements if we are running controls, i.e. blank buffer solution and comparing different surfactants, rather than absolute quantification of single independent surfactant?
Thank you.
Abhijeet Lokras
This device is used in our laboratory. I think that your doubts are in vain. Such a mistake is acceptable. You don't seem to appreciate the probabilistic error. To do this, you need to make 7 measurements, discard the largest and smallest. Divide the difference in distribution in half. Add + and minus scatter. More errors can be due to underestimation of surfactant solubility, superposition of monomolecular layers ...
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