Has anyone extracted plasmid DNA from the Dmp1-GFPtopaz plasmid?

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Hanna Alalam

I think your issue might be with the antibiotic concentration. It is low which might cause you to be extracting from cells that already lost the plasmid. Increase the concentration to 100ug/ml and try again.

Abhijeet Singh

I am not sure why you used Carbenicillin. The plasmid has Ampicillin resistance.

Abhijeet Singh carb can be use instead of ampicillin for selection for AmpR it is just more stable.