Besides the commercially available in-vitro translation system such as Rabbit reticulocyte translation system,is there any detail protocol to prepare the cell lysate from any mammalian cell line??
I am working in the area of Handwriting recognition for Bangla. As there are many DL architecture, I am confused which one to select.
15 February 2021 4,996 1 View
I want to know how we can find "call for papers" in a special issue? Does it increases chances of quick publication and acceptance of research paper?
09 February 2021 3,748 5 View
Hi everyone, I am looking for fluorescence expression vectors for expression in gram-positive bacteria present in the skin microbiome. I need to tag them with a fluorescence marker and observe...
06 February 2021 2,566 4 View
Astaxanthin is a keto-carotenoids having reddish color. It occurs naturally in certain algae and causes the pink or red color of different seafood especially salmon, shrimp, trout, and lobster....
29 January 2021 7,861 2 View
Hello Everyone! I have prepared liposomal formulation using Rotary Evaporator method followed by dialyzed the liposomal Suspension in Phosphate buffer to remove the un-encapsulated drug. After...
25 January 2021 7,752 4 View
I want to have a optical system where, I focus a beam onto the BFP of a microscope objective. Unfortunately, I always have a strong Back Reflection from the objective. Can anyone suggest me some...
18 January 2021 2,062 8 View
I need to preserve broth culture of wet fungus spore for my further research. Can any one suggest me any solution.
18 January 2021 4,756 2 View
I have done a docking study by using mcule.inc web-service. I got the .pdb file of my protein-ligand complex from there. As I am using a free student account, I don't have access to more...
14 January 2021 2,321 2 View
I am working on virus production through bioreactor,my concern is regarding ,no increase in virus titer after 24 hour of seeding for harvest Usually we seed the fermenter and incubate for 72...
05 January 2021 3,860 2 View
Hello everyone This is regarding finding elastic and transport properties of a material using the above mentioned softwares. Right now I am using Quantum Espresso to find out the structural,...
28 December 2020 6,767 1 View
As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...
23 February 2021 3,175 3 View
Is it possible to implement FBM in a commercial FEA software package, such as FEMLAB, COMSOL, or open source software like Moose?
22 February 2021 2,968 1 View
I am trying to set up a cortisol EIA using mouse anti-rabbit IGg as coating and a rabbit cortisol antibody as secondary ab. I am searching for a sensitive and specific cortisol Ab made in rabbit
20 February 2021 3,366 5 View
Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...
08 February 2021 7,285 1 View
Hello, I have recently run a western of Vero cells for ACE2. I detected ACE2 at the correct size, but it presents as a double band. The sample was prepared by lysing cells in 1X Laemmli...
07 February 2021 6,973 3 View
Hello, I am western blotting SARS-CoV-2 spike protein from lentiviral using an antibody against S1. To prepare samples, I mixed concentrated lentivirus 1:1 with 2X Laemmli buffer and boiled...
07 February 2021 3,643 4 View
Dear colleagues I have the following questions concerning the safety of mRNA vaccines targeting the spike protein of SARS-Cov2: 1) The vaccine elicits antibodies which block the peptide domain...
04 February 2021 721 6 View
Hello, I hope to insert a gene into mammalian cells in culture using CRISPR/Cas9 and an HDR template. Will insertion of a 1 kilobase gene cause chromosomal abnormalities and effect cell...
31 January 2021 2,516 1 View
Hello, I am conducting a sandwich-type ELISA. It is to see if small molecules can interfere with a protein-protein interaction. As this interaction would occur in the human body, may I heat...
31 January 2021 4,271 3 View
I want to find a database(s) or a method where I can find and bulk download all the rabbit MHC genes and domain annotation. In humans using Ensembl, all MHC genes have names and domains but in...
28 January 2021 7,722 2 View