Jyoti Roy

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Questions related from Jyoti Roy

Has anyone expertise in making HeLa cell lysate to be used for In-vitro translation??

Besides the commercially available in-vitro translation system such as Rabbit reticulocyte translation system,is there any detail protocol to prepare the cell lysate from any mammalian cell line??

15 July 2020 3,049 0 View

Can any one suggest: What should be the exact procedure to be followed in western blot for 100 kD protein?

I have used the following procedure and getting the expression of endogenous control Beta actin (42 kD) but unable to get expression of desired proteins (100 kD and 97 kD): 1. 10% resolving and 5%...

07 August 2015 8,507 9 View