My colleague and I have generated the triple gene deletion strain (BY4742 delta gpd1, delta gpd2, delta dld1). At first, it grew well on both solid and liquid YPD media or even in 2X YPAD media. This suggests that at least the gene deletion did not impair growth directly. However, after sub-cultivation for 2 generations, it lost growth ability in liquid SC, YPD, and 2X YPAD media, but still be able to grow on solid YPD media.
Could anyone give me some advice ?
Thank you very much for your suggestion in advance.
Additionally, this strain was also capable of producing D-lactic acid
HI Akaraphol Watcharawi, Iam having the same problem with a transformed yeast strains. They growth excelent in solid media but the same media liquid the OD is 0.06 maximum. What could be the problem???
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