Hi!!! We have bought Ethylene glycol chitin with the hope of working with a more soluble chitin substrate in the enzymatic assays. We tried to prepare the stock (0.5mg/ml) using water+sonication but it was not soluble. We have used other approaches as increasing the temperature (even 95ºC) or decreasing pH (up to 5)...
Has anyone experience in preparing a stock of Ethylene glycol chitin?
Thanks a lot,
Enea
Dear Enea,
Maybe you don´t have ethylenglycol chitin polymer. The main quality of this polymer is its water solubility. It readily form a clear transparent solution in water once dissolved.
However, before talk with your provider you can try to put hot water (60°C) under magnetic stirring and add slowly the polymer trying to not to form aggregates difficult to dissolve. Each amount of polymer have to be dispersed before adding next amount of polymer and left the mixture stirring at this temperature for 2 hours. If nothing happens you can try to add a drop of NaOH to this solution. If this dissolved the polymer maybe you will have a carboxymethylated polymer instead glycolchitin.
You can do a FTIR spectra and compare with previously published data on this polymer also.
Hope it will help you,
Best regards,
Gustavo
- Badges
- Science topic
- Similar topics
- Engineering
- Materials Engineering
- Powders