Enea Sancho-Vaello

3 Questions 2 Answers 0 Followers

Questions related from Enea Sancho-Vaello

Does anyone knows what the uneven line is?

Hi everyone! I performed two chemical cross-linkings with my peptide using glutaraldehyde and BS3, as cross-linkers. After that, I stained the gels with the silver staining kit (Invitrogen). In...

26 September 2023 9,356 0 View

How could a transcriptional factor purified as inclusion bodies (by using urea) exhibit a ratio 260/280>1?

I am working with a very unstable transcriptional factor. I use DNAse in the lysis buffer and I purify it as inclusion bodies, by using urea and 1M NaCl. After purification, I buffer exchange the...

06 February 2021 2,860 3 View

Has anyone experience in preparing an Ethylene Glycol Chitin stock?

Hi!!! We have bought Ethylene glycol chitin with the hope of working with a more soluble chitin substrate in the enzymatic assays. We tried to prepare the stock (0.5mg/ml) using water+sonication...

04 May 2016 9,873 1 View