How could a transcriptional factor purified as inclusion bodies (by using urea) exhibit a ratio 260/280>1?

I am working with a very unstable transcriptional factor. I use DNAse in the lysis buffer and I purify it as inclusion bodies, by using urea and 1M NaCl. After purification, I buffer exchange the...

05 February 2021 2,530 3 View

Optimal potassium to magnesium ratio in soils ?

Hi, After some research, I have found recommended soil potassium to magnesium ratios ranging from 0.25 to 0.7 in agricultural soils (ratios calculated from CEC concentrations). Which one do you...

26 July 2020 5,412 8 View

How to study the crystal structure of nano ferromagnetic materials? How to arrive at a suitable doping agent?

What are the books/articles to study the crystal structure of nanoferromagnetic materials?

31 March 2020 4,195 4 View

Hi, I'd like to stain mitochondria with TMRM in situ, anyone has a protocol?

I would like to see by confocal microscopy the activity of the mitochondria potential membrane using TMRM. The problem is that I can't find a way to stain at TMRM in situ. I can process the...

04 March 2020 5,033 2 View

Do you know good methods to describe the internal morphology of insect larvae?

I am looking for good methods to describe the internal morphology of hymenoptera larvae stages. If you know a related technique or protocol, I would appreciate if you indicate it.

22 February 2020 1,359 3 View

What is the maximum temperature the soil can reach in a ferruginous rock field?

I searched online but I could not find any answer to it. Does anyone ever measures it or knows a work on this topic with that info? Thanks :)

10 July 2019 1,415 3 View

What are the books/papers to learn about quantum confinement in 1D, 2D and 0D nanostructures?

What are the books/papers to learn about quantum confinement in 1D, 2D and 0D nanostructures? What are books/papers to know more about nanoelectronic devices?

07 January 2019 6,464 8 View

How to proceed with molecular dynamic simulations in nanoscale?

How to commence simulations in nanoscale? In which part of the code do we introduce the nano dimensions?

15 December 2018 9,749 6 View

Why do I obtain false positives in Datsenko strategy (red recombinase)?

Hi there! I am trying to obtain a knock-out using Datsenko strategy. I am working with pKD4 (as a template for PCR) and the pKD46 vector. Once I obtained the linear DNA (using primers with 50 bp...

09 December 2018 2,676 10 View

How to add a journal ?

23 October 2018 7,341 89 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View

How to calculate battery consumption for IoT device?

Hi, everyone I'm working on a privacy-preserving algorithm that will apply to IoT devices. So, I need to measure the power consumption by implementing this algorithm. Any suggestion, advice,...

25 February 2021 8,749 6 View

How can plant phytochemistry be included in discussions about whole plant traits?

I am using the concept of plant functional traits (Cornellisen et al., 2003) in a paper. Considering descriptions for whole plant traits (life history; life form; growth form; plant height;...

24 February 2021 6,831 5 View

How vulnerable is water and wastewater infrastructure to cyber terrorism in an age of IoT?

Im looking for any papers, reports,articles, links etc on information on How vulnerable is water and wastewater infrastructure to cyber terrorism in an age of IoT.

24 February 2021 2,750 4 View