To our understanding developing an endothelial capillary network is essential for bone tissue regeneration and defect compensation. So it is not sufficient to just augment single cell lines.
Dolores Blauth voted my question up, that's very kind and nice :-) However, I am still missing any comment. Does that mean nobody ever tried to do co-cultures of bone tissue?
Any comments are really welcome, especially to why co-cultures were not done, if so; if yes, how about the results?
Axel
Sean, thank for your reaction. According to Erica Scheller in bone growth and remodeling there are appr 30 different cell types actively involved in vivo. Co-culturing at least endothelial cells together with bone precursors seem to be more promising than just isolating single cell lines out of a biopsy and discard the others, regarding successful re-implantation.
What were your results with respect to the viability of your cell conglomerate? Did you use human demineralized bone matrix as a scaffold?
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