If yes, could you please shed some light on what are these cell types and in what kind of in vivo conditions (disease, non-disease, inflammation, etc) have you encountered them? References would help. Thank you!
Gr1 binds to both Ly6C and Ly6G. Ly6G+ cells are neutrophils. Ly6C+ cells include a range of cell populations. Most of the CD11b-Ly6C+ cells you're seeing will be CD8+ T cells and, in B6 mice at least, pDCs. There may also be some gd T cells and NK cells in there as well.
Hey Almin,
In my three years of using these markers, I have never seen the CD11b-Gr1+ population. Have you tried staining with a different CD11b antibody (perhaps in a different channel) just to rule out issues with the Antibody?
Ben, thank you for comment. Do you perhaps have a reference to that, in particular to your comment on the B6 pDCs?
(How would you interpret if this Gr1+CD11b- population is also CD11c-?)
This is one of the better pDC papers (http://www.ncbi.nlm.nih.gov/pubmed/19570827), though it's not the first to show Ly6C expression by pDC. That particular observation seems to be well-appreciated. I still think that the most abundant CD11b-Ly6C+ cells are CD8+ T cells (http://www.ncbi.nlm.nih.gov/pubmed/21308682). Since T cells do not necessarily express CD11c, I would say that the CD11c- cells are probably still CD8+ T cells.
To clarify with an older reference: Ly6C expression by pDC is described in one of the original pDC papers: http://www.ncbi.nlm.nih.gov/pubmed/11713464
I have just noticed that pDC do not stain well for Gr-1, despite staining very well for Ly6C. Possible that Gr-1 doesn't bind all Ly6C+ cells?
Thank you Ben for your answers. I have indeed noticed that most of the Gr1+ CD11b- cells are Ly6C+ LyG- so it is likely that they could also be positive for CD8. Will definitely check that and let you know. And Simon I have used FITC and Cy5 - CD11b and both give similar results so I don't think it is an antibody issue. But thanks for your input. Will keep you guys posted!!
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