Why to use DNA isolation kit. Try CTAB method for DNA isolation. I have used this method for isolation of DNA for variety of fungi. 

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I'm also doing fungi DNA. There's a simple method you can try. What you need to prepare is just 1x Tris-EDTA (TE) buffer. Pick small amount of fungi culture using sterile bambo stcik/toothpick, transfer into 96-well plate contain 100ul 1x TE buffer. then incubate at -80C for overnight. then after overnight, take out the well and thaw at room temperature. Heat shock the well at 60C and shake for 1min. You can prepare the PCR mixture earlier. Aliquot 28ul of PCR mixture + 2ul of crude DNA into PCR tube, then PCR, electrophoresis and visual.

Thanks Padder, I am currently using CTAB with good result.

I tried the Kit trying to reduce the time and energy due to the quite many isolayed I need to extract from.

Thank u.

Thanks sir Mr Oke, I have sent an email to them.

@Jamilah Hassan, this method is very interesting, pls can you send me a more detailed procedure? I will like to use it. My email is [email protected]

@Jamilah Hassan: How does the crude DNA can be store? for few weeks or months?