Has anyone encountered issues for infecting mice with Streptococcus pneumoniae?

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Is it harmful to breed for Cre Homozygosity initially?

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Nurul Adhwa Rahman

What are your issues? Pneumococcal animal models have already been performed and I have not read any published issues with regards to this. You can read studies by Iovino et al. (2013 until 2018).

Demba Sarr

The mice are not getting sick!

Ziyi Guo

Intranasal infection is not very pathogenic. Intratrachael infection can be better. I used D39 strain and did intratrachael infection C57BL/6 with CFUs 10^7. All the mice died within 4 days.

Thank You Ziyi. This is very helpful!