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We are using the T4 strain for our intranasal infections (40 microliter in PBS) with CFUs above 3000000 but never got mortality of C57BL/6. Any comment?
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I have been doing immunofluorescence on paraffin-embedded tissue sections and I was wondering if we can still dehydrate the fluorescence labelled tissue sections and mount them exactly like we do...
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I have been using PBS but I don't get staining in some cases.
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