Prompt research in the direction of "deprivation and adolescents" please?

01 February 2021 6,401 3 View

How to combine and exclude processed images on ImageJ?

Hi everybody. I have already created two different Analyze Particles image results (with a previous thresholding process for each one) from the same primary image, with their respective area...

11 August 2020 9,428 2 View

Any ideas on where to look to for organization theory that speaks to why people join organizations?

I am studying place attachment. Place attachment is usually examined by looking at place and the emotional connection individuals have with place. However, I am interested in learning about how...

11 June 2020 9,483 7 View

How does the stress-strain diagram change during continuous dynamic recrystallization ( CDRX) in steel?

It is known that during discontinuous dynamic recrystallization (DDRX) the stress-strain diagram has a stress peak . And how the diagram changes during continuous dynamic recrystallization (CDRX)...

20 March 2019 3,676 4 View

Negative correlation interpretation?

How would you describe the relationship between two scores with an r obtained of -.312, with a p value = .000?

25 November 2018 6,909 7 View

Can we use Pearson's r for determining the relationship of two variables with instruments using the likert scale?

Our thesis research has the following questions: 1. What is their level of (variable a)? 2. What is their level of (variable b)? 3. Which of the five components of (variable b) relate to...

04 November 2018 2,825 4 View

Does anyone have a copy of the questionnaire and all the psychometric properties of workplace deviance?

This particular scale is needed for my thesis. Thank you very much.

09 September 2018 6,117 3 View

How can I change the y-axis scale of R grofit graphs?

I am trying to plot a Gompertz growth model using grofit. However, ylim in plot() for grofit package is not working. How can I fix it? The code I am using is: library("grofit") data

28 August 2018 3,509 5 View

Recode some items or not?

In my survey, I asked the type of emotions felt by respondants during an event (positive & negative); 4 adjectives/type (Happy, satisfied, excited, dynamic & bored, angry, annoyed, asleep)...

21 July 2018 3,432 2 View

If I have a concetration 100ug/ul, How do I do for obtain 50ng to PCR?

24 June 2018 5,963 7 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View