Tatiana Zúñiga Burgos

5 Questions 6 Answers 0 Followers

Questions related from Tatiana Zúñiga Burgos

Cell fixation for DAPI staining in Chlamydomonas reinhardtii?

Hello Everyone, For a time-course experiment, in which I need to collect samples everyday for bio imaging using DAPI staining, I'd like to know if there is a way in which I can fix my cells in...

26 August 2020 593 4 View

Does anyone know if Chlamydomonas reinhardtii cw15 arg- grows on nitrate (NO3-)?

Hello everyone, For my research I need to know if Chlamydomonas reinhardtii cw15 arg- grows on nitrate or not, to see if I can do some tests changing the nitrogen source. However, I cannot try...

26 March 2020 1,106 3 View

Has anyone done a two plasmid simultaneous transformation in a chemically competent E. coli?

I have implemented NEB protocol for E. coli BL21(DE3) competent cells in order to transform two plasmid at the same time, but i just have transformed one plasmid in E. coli. Thanks!

06 August 2017 2,861 6 View

Spectinomycin (LB medium) concentration

Hello :). I have to put my plasmids (dh5alpha) in LB medium containing spectinomycin. Whar i´m wondering is which is the right antibiotic´s concentration due to the fact that my plasmid is a low...

31 May 2017 1,679 4 View

What is maximum amount of plasmids, i could insert in a host cell? Why? Also where can i find some useful information about plasmids compatibility?

I`m planning my experiment and have three to four plasmids to insert in my host cell, but don´t know if it is possible.

18 March 2017 7,345 4 View