Tatiana Zúñiga Burgos

6 Questions 6 Answers 0 Followers

Questions related from Tatiana Zúñiga Burgos

Cell fixation for DAPI staining in Chlamydomonas reinhardtii?

Hello Everyone, For a time-course experiment, in which I need to collect samples everyday for bio imaging using DAPI staining, I'd like to know if there is a way in which I can fix my cells in...

26 August 2020 650 4 View

Does anyone know if Chlamydomonas reinhardtii cw15 arg- grows on nitrate (NO3-)?

Hello everyone, For my research I need to know if Chlamydomonas reinhardtii cw15 arg- grows on nitrate or not, to see if I can do some tests changing the nitrogen source. However, I cannot try...

26 March 2020 1,167 3 View

Trace elements solution for minimal medium??

Hi :), I have found lots of protocols for both the preparation of the trace elements solution and minimal medium for PHB production by Ralstonia eutropha H16, however these protocols are not...

09 August 2017 1,279 1 View

Has anyone done a two plasmid simultaneous transformation in a chemically competent E. coli?

I have implemented NEB protocol for E. coli BL21(DE3) competent cells in order to transform two plasmid at the same time, but i just have transformed one plasmid in E. coli. Thanks!

06 August 2017 2,951 6 View

Spectinomycin (LB medium) concentration

Hello :). I have to put my plasmids (dh5alpha) in LB medium containing spectinomycin. Whar i´m wondering is which is the right antibiotic´s concentration due to the fact that my plasmid is a low...

31 May 2017 1,756 4 View

What is maximum amount of plasmids, i could insert in a host cell? Why? Also where can i find some useful information about plasmids compatibility?

I`m planning my experiment and have three to four plasmids to insert in my host cell, but don´t know if it is possible.

18 March 2017 7,400 4 View