I’m exploring why serology underperforms in cutaneous leishmaniasis (CL) versus visceral leishmaniasis (VL). Does anyone have published studies or personal experience comparing antigenicity/humoral responses between CL species and VL using any kind of antigens (recombinant antigens, whole-parasite lysates, membrane fractions, LPG, excreted–secreted antigens)? What about IgG titers/subclasses, avidity, antigen breadth, memory B cells? any tips to reduce cross-reactivity?
Please share references, protocols, pitfalls, and any datasets.
Hi Elfadil, Yes, there are several studies on this topic. In general, the humoral response against CL is weak. Antigens that perform well for VL typically show low sensitivity for CL. For example, we tested our VL antigen with Brazilian CL samples and observed no reactivity (0% CL sensitivity). Similarly, when tested against Sri Lankan CL, the antigen showed only 10% sensitivity, despite CL being caused by the same L. donovani species [doi: 10.1038/s41598-019-46283-9].
Are you working with a CL-specific antigen, and on which platform are you detecting the cross-reactivity?
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