Has anyone carried out a protease assay using EnzChek?

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How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

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How to constrain genetic algorithm input u?

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The Role of Town Planners in CAV Use?

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Why are the modelled and circuit system different in response for controlled buck converter?

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How can I interpret a ratio variable?

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What is best technique to quantify necrotic cell death of specific cell populations?

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Why do some full text requests not allow me to respond to the requesting person?

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Spectrabase no longer has overlay function?

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Why is Statement of Problem one of the most challenging part of academic research? Why do supervisors pay more attention to it instead of methodology?

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Follow up tests to explore interaction effects in a multiple logistic regression: can someone help explain my contradictory results?

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MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

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MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

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fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

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fatal error

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fatal error

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fatal error

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Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

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What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

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How to repeat an operation on an atom selection for multiple times in PyMol?

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New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

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Joshua Gustafson

What was in your negative control well? Any of the fluorescent substrate? What kind of plate are you using (wells, plastic/glass/opaque bottom, clear/opaque wells, etc.)?

Robert Szczesny

I used a RediPlate EnzChek red (BODIPY TR-X dye) kit and a TECAN reader to test some bacterial proteases. It took 10 to 20 runs to find the right conditions for this particular combination of clear flat bottom tube and battered reader. To find the right filter set and define all appropriate parameters on the reader I diluted the substrate in eight steps from 0 to 2.5µM (according to the manual) and added Proteinase K to digest the casein. By adjusting the number of flashes, flash duration and gain value I was able to quickly find the right conditions. Good luck!