Macrophages in culture not growing/ dying or detaching . Why ?

Hello, I am an undergraduate student doing my dissertation, where I isolate PBMCs from leucocyte cones in order to plate monocytes and allow for macrophage differentiation. Although the numbers...

09 February 2021 5,334 8 View

HT22 purchase in Greece?

Hello everybody, I am looking for a specific cell line HT22 but I haven't found any company that could import it in Greece. Has anyone faced that problem before? Is there any alternative way that...

01 April 2019 1,917 3 View

Hello, Can anyone help me use krona tool for visualising my NGS data?

My files contain data in percentages or in counts.Does Krona tool need bioinformatic language to be used? Thanks in advance.

27 January 2019 1,758 2 View

How can we estimate how much is the sensitivity of a Chi Squared test to the number of classes?

Should we run the experiment with different number of classes and see if there is any difference?

23 August 2018 3,780 2 View

Why do I need to wash the entanol from ethanol stored samples with Tris-HCl before extracting DNA?

I worked at a laboratory and before extracting DNA, I washed of the ethanol with Tris-HCl, in order to get rid of all the ethanol before starting the extraction process, but I did not understand...

02 February 2018 9,725 1 View

Yeast fermentation medium recipe??

Can anyone please explain what is a fermentaion medium and a yeast suspension and how to make them? I have attached the paper that these two are mentioned and I have highlighted them in the...

03 January 2018 3,151 2 View

How can i dilute carvacrol (oil) ?

I have read in some papers that carvacrol can be diluted in Tween 80 (1%) and distilled water. I have tried this but carvacrol wasnt fully dissolved. After that i tried to dissolve carvacrol in...

09 August 2016 6,070 7 View

How I can get the Organizational Commitment Questionnaire?

Dear Dr. Meyer, I would like to inform you that I am a student candidate for a PhD in Cyprus University of Technology and my subject relates to job satisfaction, leadership style and commitment of...

27 March 2016 6,828 15 View

Is a standard curve required for each assay when we use an elisa kit?

Does anybody know if i can use some wells ( about 10 wells)of the TNF-a elisa kit in order to try my samples? I have not used a kit before and i wonder whether i can use some wells without running...

25 March 2016 5,274 17 View

What kind of cells should I to use in order to study the process of inflammation ?

30 November 2015 7,494 12 View

Good sites to recruit survey participants on a grad school student budget?

Any good recommendations for participant recruitment for online surveys on a grad school student budget?

02 March 2021 4,293 4 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

How to compute the internal consistency of a scale with ordinal items?

Hi community, I am trying to determine what is the best way to calculate the internal consistency of a newly developed self-esteem scale. I am working together with two other researchers on the...

28 February 2021 1,369 5 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

Can impedance of antenna be less than 50 ohms?

I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...

27 February 2021 4,218 7 View

How can I set up a good fit SEM model in AMOS?

I am trying to make an SEM model in AMOS for my dissertation but having some trouble with getting a good fit. I am looking for relationships between self-efficacy reported on a Likert scale and...

26 February 2021 8,642 4 View