Strategies for short hairpin RNA interference cassettes?

In your experience, how much does the distance between the U6 promoter and the annealed -short hairpin - oligonucleotides affect the RNAi cassette expression? How to clone a shRNA sequence...

16 January 2021 9,418 2 View

How should private health-care systems be improved to guarantee everyone's right to health?

COVID-19 pandemic has shown big social inequalities in countries with private health-care systems. Governments must pay greater attention to the right to health, that every human being must have,...

31 May 2020 7,357 23 View

I'm trying to set up a TR-FRET experiment in my lab but I'm having some troubles. Could you give some tips?

I'm working with an his-protein and I'm trying to obtain an IC50 between the protein and the biotinylated substrate and a known competitor. But the experiment is not working. I will add the...

20 April 2020 4,485 3 View

Problems with Google Scholar. Do you know how to fix it?

Dear All I am having some problems with google scholar. Some citations to my papers simply disappeared. I know that the number can be reduced when two different versions of a paper are...

01 July 2018 873 60 View

Is organic chemistry predictable?

It seems like organic chemistry consists of learning a bunch of reactions, but is there any qualitative set of rules for predicting the way a set of organic reagents will react? I know that...

30 May 2017 3,980 3 View

Are there any recognized / respected certificates for R programming or data analysis ?

Thinking about online and MOOC-type certificates for R programming and data analysis, are there any that are recognized and respected by potential graduate schools and employers? I guess if people...

11 March 2017 8,044 3 View

Hi all, what is the best method to determine the onset of bird migration and the end (day arrival), using GPS points?

There is a clear evidence in my data, but I would like to have also a statistical evidence

17 September 2016 7,140 4 View

Is there a Cre line specific for the immune system?

22 June 2015 7,815 2 View

Is it possible to conduct AFM electrical measurements (such as EFM and KFM) in liquids?

I was wondering is any of the above techniques can be used for measurements in liquids. Thank you!

19 January 2015 3,178 3 View

Do you know some organic solvents which are capable of solubilizing the KMnO4 without oxidation?

I am developing a protocol for controlled oxidation of Cobalt Nanoparticles in solution. The nanoparticles are dispersed in hexane, so, I must find a solvent which is miscible with the hexane.

19 January 2015 5,805 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View