I understand this can be done rather easily with an appropriate linker molecule (NH2-PEG-SH); which should bind to the COOH group on the Fc region of the IgG antibody and later form a Thiol bond to the gold surface with the idle SH group. Still, it sounds a bit trickier.
Could anybody recommend a protocol for this approach, or suggest a scientist with published protocols on this issues?
I´m having a hard time finding protocols to inmobilize IgG antibodies to a gold surface. The other approach is an aptamer/primer based probe (Plaxco, Rowe, et. al, 2011).
Kindly share your experience and oponions on this matter.
Best
Pedro
I've never worked on gold surface, but I did some coupling on other materials. I had my best results modifying the surface and then attaching the Ab on it (and not doing the opposite). In this way you reduce protein manipulation (it's always a risk, as you don't know if you are compromising its 3D folding) and usually it's a lot easier to work with a surface than with a macromolecule.
You can find a lot of protocols and examples on "Bioconjugate techniques" (Hermanson GT).
Excellent suggestion Emmanuele, thank you. It makes perfect sense!. I'll start reading and report back :)
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