Books on Monte Carlo methods?

Hi everyone. I took a basic course on Markov Chains, and know a little about Monte Carlo Stimulations and Methods. But I never got to the part of spreadsheets. If anyone can help direct me to a...

13 February 2021 5,261 3 View

(ORGANISATIONAL FACTORS ) request for study instrument?

i am currently conducting a study to assess bullying behaviors on student nurses during clinical placement . My objective is to explore organisational factors that influence bullying behaviours at...

25 January 2021 8,676 1 View

How to express accuracy of the neural network where the dataset has not been split into two, training set and test set?

In use, the weights obtained are considered with the greatest accuracy when the data set is divided, because the weights are initialized at random and the training data is swapped at random...

19 January 2021 5,714 4 View

Why is the study of neuroethology critical to brain research?

One of the assumptions behind neuroethology is that the brain and the genes that are involved in its development are shaped by the success of the behaviors exhibited by a species so that the genes...

04 January 2021 9,763 6 View

Is it always mandatory to report and test the validity and reliability of a newly developed questionnaire?

Hello everyone! Currently, I am doing an observational, cross-sectional study on knowledge, atittude, and practice towards thalassemia in Indonesia. My area of interest is in population studies....

16 December 2020 3,725 16 View

Will automated killing diminish the error rate by police?

10 December 2020 5,548 3 View

Assay with absorbance/fluorescence readout?

I'm setting up a liquid handling robot and need a simple assay to test it. Ideally I would like to mix 3-4 reagents and have a absorbance/fluorescence readout on a plate reader. It's also possible...

11 November 2020 7,645 3 View

What are suggest articles or books relating to Game Theory?

I am looking for a good couple of reads on game theory, maybe with an historical context. Anything like random walks, or Marvok Process is fine or even, Colonel Blotto, and Prisoners Dilemma. I...

30 July 2020 710 3 View

How to escape local minimum in the training of Convolutional Neural Network?

I am trying to train CIFAR10 dataset on CIFAR10 architecture on MATCONVNET without gpu but my objective function is not decreasing down to 1,665. What may be parameters to tune while considering...

16 June 2020 4,700 8 View

Data transformation to fit a beta regression?

Dear all, I'm intended to model the effects of a number of categorical factors (season, sex, age) on the proportion of lean birds using a beta regression (R-package 'betareg'). The dependent...

12 June 2020 826 2 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Which transfection reagent is the best for plasmid transfection of MDA-MB-231 cell line, Lipofectamine based or other reagent suggestion?

I try to transfect MDA MB 231 cell line with a plasmid by PEI, unfortunately, the rate of transfection is low, and now I wanna find and order the best transfection reagent for this cell line and...

19 February 2021 828 6 View

In what ways do fellow reaerchers think that mRNA vaccination may be under CRISPR systems technology application ?

There are many ways for mRNA to intervene with genome expression. This question is raised for experts in CRISPR systems. Any RNA does not stand alone within acell. There are many ways to become...

18 February 2021 9,109 5 View

SiRNA transfection efficency and results interpretation ?

i am having a problem with the transfection of 2 siRNA , one for the longer isoform U and the other one of the sliced isoform S that is also the only transcribed. i am trasfection siRNA with a...

11 February 2021 8,145 3 View

How can I completely delete a protein on the genomic level using Crispr?

I want to completely delete or largely truncate a gene and not only achieve a functional knockout in a cell line. I can only transfect my cells with the Crispr/Cas9 sgRNA RNP and not introduce a...

08 February 2021 2,032 2 View

Do you have any experience with transfection of EBV immortalized B cells with Lipofectamine?

I know that efficiency of transfection for primary B cells is very low with Lipofectamine. But I did not try that for EBV immortalized B cells. Do you have any experience with transfection of EBV...

05 February 2021 4,159 1 View

Choise of cell line for neurobiology?

I am working with myelin-related proteins and interested to do cell experiments with my target protein in cells. I have created lentiviruses for my proteins, and mutants and also CRISPR set-up for...

02 February 2021 8,083 1 View

Does gene insertion via CRISPR/Cas9 result in chromosomal abnormalities?

Hello, I hope to insert a gene into mammalian cells in culture using CRISPR/Cas9 and an HDR template. Will insertion of a 1 kilobase gene cause chromosomal abnormalities and effect cell...

31 January 2021 2,516 1 View

Does anyone has a protocol for transfecting AML12 cells?

I am optimizing a CRISPR/CAS9 system in vitro before using it in vivo. I would like to transfect AML12 cells with my pAAV-sgRNA-vector to validate editing efficiency before generating virus.

26 January 2021 8,733 2 View

Intranasal immunization with a DNA-vaccine - any advice about transfection reagents or adjuvants?

Hallo everyone! My lab is engaged in a project on creatin a candidate DNA-vaccine. As a spin-off of the main work we want to try intranasal immunization of mice with our experimental DNA-vaccines....

20 January 2021 8,221 2 View