If cells are incubated at physiologically-relevant oxygen levels (in my case, 5%), room air is hyperoxic. The longer the cells are out of optimum conditions, the more they should be affected by routine cell handling. Has anyone looked at the time factor for room-air handling?
Hi Alicia,
According to the data of Gewandter et al., published in Free Radic Biol Med. 2009, 47(12): 1742–52., human lung adenocarcinoma cell lines A549 and H1299 exposed to room air or hyperoxia for 36 hours (and then treated with 50ug/ml cyclohexamide), all showed time factor for room-air handling (determined by declining of several critical cell parameters) about 36-48 hours.
Best wishes,
Ilya
I did a limited comparison on human chondrocytes between a glove box apparatus and a low oxygen incubator both at 5% O2. Normal feeding regimes exposed the cells that were in the incubator, rather than the glove box, to room air and non-physioxic media for less than 20 minutes (depending on how quickly you estimate media to equilibrate with the incubator air). There was no significant difference in the cell accumulation of glycosaminoglycan, hydroxyproline or DNA which are dependent on a low (physioxic) oxygen tension.
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